Melting Curve Analysis for Rapid Detection of Topoisomerase Gene Mutations in Haemophilus influenzae
نویسندگان
چکیده
منابع مشابه
Melting curve analysis for rapid detection of topoisomerase gene mutations in Haemophilus influenzae.
We established a real-time PCR assay with melting curve analysis to rapidly genotype quinolone resistance-determining regions (QRDRs) of gyrase A and topoisomerase IV genes in Haemophilus influenzae. This assay is a useful tool for the detection of fluoroquinolone resistance and for the early detection of preexisting QRDR mutations.
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Hearing loss is a common birth defect worldwide. The GJB2, SLC26A4, MT-RNR1 and MT-TS1 genes have been reported as major pathogenic genes in nonsyndromic hearing loss. Early genetic screening is recommended to minimize the incidence of hearing loss. We hereby described a multicolor melting curve analysis (MMCA)-based assay for simultaneous detection of 12 prevalent nonsyndromic hearing loss-rel...
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ABSTRACT Background: A polymerase chain reaction (PCR) assay with primers from "hpd" gene was compared with culture for the detection of Haemophilus influenzae type b (Hib) in CSF samples from children suspected of meningitis. Methods: 300 cerebrospinal fluid (CSF) samples from suspected meningitis cases were studied by culture (Leventhal & Chocolate Agar) and PCR. Results: The latter...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2009
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01645-08